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Table 1 Mitochondrial regions tested1.

From: Glacial vicariance in Eurasia: mitochondrial DNA evidence from Scots pine for a complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor

Genomic region

Annealing temperature (°C)

PCR product size (bp)

Restriction enzymes tested

Primer source

matR (intron1)

58

550

Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI

[47]

mh05

59

1500 4

-

[91]

mh09

62

180

-

[91]

mh09'

62

900 4

-

[91]

mh33

55

900 4

-

[91]

mh35

59

Not amplified

-

[91]

mh44

59

Not amplified

-

[91]

nad1 intron B/C, primers H/I 2

55

217–248

-

[6]

nad3 intron 1

58

120

-

[92]

nad3-rps12 (i.r.) 3

58

360

-

[92]

nad5 intron 1

55

1100

Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI,

[47]

nad5 intron 4

59

900

Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI, Tru9 I

[93]

nad7 intron 1

61

1200

Sau 3AI, Rsa I, Hind III, Taq I, Bst UI, Tru9 I

[47]

SSU rRNA region V1

62

400

-

[94]

SSU rRNA region V7

50

250

-

[94]

  1. 1Targeted regions, annealing temperatures, expected size of PCR products and restriction enzymes tested for primer pairs used to amplify 15 mtDNA regions of Scots pine.
  2. 2nad1 intronB/C have been amplified (following Demesure et al [95] protocol) and tested with different restriction enzymes, only internal primers H and I of Soranzo et al [6] have shown polymorphism.
  3. 3Intergenic region.
  4. 4Multiple fragments.