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Table 1 Mitochondrial regions tested1.

From: Glacial vicariance in Eurasia: mitochondrial DNA evidence from Scots pine for a complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor

Genomic region Annealing temperature (°C) PCR product size (bp) Restriction enzymes tested Primer source
matR (intron1) 58 550 Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI [47]
mh05 59 1500 4 - [91]
mh09 62 180 - [91]
mh09' 62 900 4 - [91]
mh33 55 900 4 - [91]
mh35 59 Not amplified - [91]
mh44 59 Not amplified - [91]
nad1 intron B/C, primers H/I 2 55 217–248 - [6]
nad3 intron 1 58 120 - [92]
nad3-rps12 (i.r.) 3 58 360 - [92]
nad5 intron 1 55 1100 Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI, [47]
nad5 intron 4 59 900 Sau 3AI, Rsa I, Hind III, Mse I, Taq I, Bst UI, Tru9 I [93]
nad7 intron 1 61 1200 Sau 3AI, Rsa I, Hind III, Taq I, Bst UI, Tru9 I [47]
SSU rRNA region V1 62 400 - [94]
SSU rRNA region V7 50 250 - [94]
  1. 1Targeted regions, annealing temperatures, expected size of PCR products and restriction enzymes tested for primer pairs used to amplify 15 mtDNA regions of Scots pine.
  2. 2nad1 intronB/C have been amplified (following Demesure et al [95] protocol) and tested with different restriction enzymes, only internal primers H and I of Soranzo et al [6] have shown polymorphism.
  3. 3Intergenic region.
  4. 4Multiple fragments.