Table 2 Primer sequences used for PCR amplification reactions.
From: Persistence of duplicated PAC1 receptors in the teleost, Sparus auratus
| Â | PRIMERS | Â |
|---|---|---|
Probe synthesis | ||
   PAC1 | TM2fwd ct(g/t)(a/t)tt(g/t)tgtccttcatcctga | TM7rev ac(a/c)acaaarccctg(a/g)aagga |
Expression studies | ||
   SbPAC1A | 713T31F cgagcgatgaccttgagttag | 713T3R4 gggaggctgatgttggcgtt |
   SbPAC1B | 1CF5 catacggacacatactatgca | 1CR4 agtggccggggtctcggc |
   EGF | EGFαfwd cgctgtgacaacctgctg | EGFαrev agttccaataccgccgat |
Cloning | ||
   SbPAC1A | pcDNA3CD33T7NFwd ccgagatctagagtccgagcactgg | pcDNA3CD33T7NRev ggcgaattctcaggtggggaggctgat |
   SbPAC1B | pcDNA3CD33T7NFwd ccgagatctacaacaggtctcttcaaac | pcDNA3CD33T7NRev ggcgaattctcaagtggccggggt |
Microsatellite analysis | ||
   SbPAC1A | SpauPK713F ggagtgtgttgccgctga | SpauPK713R gtatccaaaaggctccacga |
   SbPAC1B | SpauPP1CF acccgttcatttcgcaca | SpauPP1CR ttcgccctccacacaaga |