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Table 1 Biochemical parameters of vertebrate PGI proteins.

From: Post-duplication charge evolution of phosphoglucose isomerases in teleost fishes through weak selection on many amino acid sites

 

Biochemical characters1

No. of hydrophilic charged residues

 

No. of amino acids

Hydro-phobicity (GRAVY)

Isoelectric point (pI)

Positively charged (Arg+Lys)

Negatively charged (Asp+Glu)

Difference2

Teleost fish PGI-1

      

   Fugu-1

552

-0.261

6.33

58

64

-6

   Mullet-1

553

-0.282

6.30

57

63

-6

   Smelt-1

553

-0.284

6.21

54

62

-8

   Zebrafish-1

553

-0.265

6.45

53

58

-5

   Eel-1

553

-0.359

6.36

57

63

-6

   Arowana-1

553

-0.277

6.22

56

64

-8

Teleost fish PGI-2

      

   Fugu-2

553

-0.294

6.96

59

61

-2

   Mullet-2

553

-0.265

7.85

61

60

1

   Smelt-2

552

-0.337

7.36

64

64

0

   Zebrafish-2

553

-0.304

6.82

59

61

-2

   Eel-2

553

-0.285

6.75

58

61

-3

   Arowana-2

553

-0.280

7.07

63

64

-1

Non-teleost fish PGI

      

   Sturgeon

555

-0.356

6.82

59

61

-2

   Gar

555

-0.308

6.83

59

61

-2

   Amia

555

-0.355

6.62

60

63

-3

   Bichir

556

-0.278

6.84

58

60

-2

Tetrapod PGI

      

   Toad

553

-0.226

7.68

59

58

1

   Snake

553

-0.237

8.72

62

57

5

   Chicken

553

-0.255

8.34

64

61

3

   Mouse

558

-0.294

7.75

61

60

1

   Pig

558

-0.340

7.79

62

61

1

   Rat

558

-0.285

7.38

61

61

0

   Hamster

558

-0.322

7.08

59

60

-1

   Rabbit

558

-0.292

7.11

58

59

-1

   Human

558

-0.344

8.42

62

59

3

Jawless fish PGI

      

   Hagfish

554

-0.238

7.82

57

56

1

  1. 1The predicted overall hydrophobicity (GRAVY; grand average of hydropathicity) and pI values of the PGI proteins were estimated based on the amino acid sequences translated from the cDNA sequences of Pgi genes using the ProtParam tool [49]
  2. 2Differences in number of positively and negatively charged residues in each PGI protein