NJ tree based on metazoan Zic-related aa sequences. NJ tree of Zic was drawn by MEGA3  by using Zic ZF core sequences (double underlined region in Fig. 4). The evolutionary distances were determined using the JTT matrix . The internal labels indicate the percentage value of bootstrap test (1000 replicates). Scale bar represents evolutionary distance in substitutions/aa residue. Classification of Zic genes according to a previous study  is indicated by broken line and arrows.