Multiple alignment of Pax6 aa sequences. The sequences corresponding to Pax6 PD (A) and HD (B) were aligned. Dots indicate aa residues identical to those of the top line (Drosophila _toy).Dashes indicate artificial spaces inserted by the alignment program. + indicates the residue that interact with DNA . Horizontal shaded lines indicate the Dicyema sequence. Vertical shaded lines indicate aa residues strongly conserved in Pax6 subfamily, but not in the others.Open boxes represent the positions of introns. The phases of introns are (a), 0; (c), 1; (d), 0. Open circles indicate animals in which intron positions were examined.