Syntenic conservation, gene structure, and splicing variants of PRDM7 and PRDM9. (A) Comparison of the syntenic blocks around PRDM7 and PRDM9 in vertebrates. Each chromosome is depicted in a different colour, except for the genomic regions around the PRDM7-9 genes that are all cyan. PRDM7 and 9 are represented as grey blocks. The chromosome number in the corresponding genome is provided. Dashed lines correspond to regions of break of synteny. Abbreviations: Hs, Homo sapiens; Pt, Pan troglodytes; Mma, Macaca mulatta; Mm, Mus musculus; Rn, Rattus norvegicus; Gg, Gallus gallus. (B) Gene structure of PRDM7 and PRDM9. Since for chimp and macaque no mRNA sequences are available, the human PRDM7 and 9 were used as templates for gene predictions. In chimp, the intron putatively gained by PRDM7 is composed of eight repeats. In the genomic regions corresponding to chimp PRDM9, there are four additional Zn-Fingers, which are reported in black because there is no evidence for their transcription. The dashed lines represent regions of gaps in the genome assembly. In rodents, the last intron is longer and not in scale; the corresponding length is reported in brackets. (C) Splicing variants of human PRDM7 and PRDM9. The grey lines represent the genomic regions of segmental duplication. The corresponding chromosome number, chromosomal coordinates and direction of transcription are given. For PRDM9, the splicing variants present in the database are shown. For PRDM7, both the database transcripts and the isoforms detected in this study are reported together with an in-silico gene prediction obtained by using the PRDM9 long isoform as template.