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Table 2 Adenylation domain specificity codes

From: Recurrent adenylation domain replacement in the microcystin synthetase gene cluster

Organism Strain Adenyl -ation domain Binding pocket LC-MS
    235 236 239 278 299 301 322 330 331 517  
Anabaena sp. 90 McyB1 D V W F F G L V D K L-Leu
Anabaena sp. 18B6 McyB1 - - - S - - - - - - L-Arg
Anabaena sp. 66A McyB1 - - - S - - - - Y - L-Hty
Nostoc sp. 152 McyB1 - A L - - - - I Y - L-Leu
Nostoc sp. IO-102-I McyB1 - I K N - - A I V - L-Leu
Hapalosiphon hibernicus BZ-3-1 McyB1 - - - - - - - - - - L-Leu
Planktothrix agardhii NIVA126/8 McyB1 - A L - - - - - - - L-Arg
Planktothrix agardhii 213 McyB1 - A L - - - - - - - L-Arg
Microcystis aeruginosa PCC 7806 McyB1 - A - - L - N N V - L-Leu
Microcystis viridis NIES102 McyB1 - - - T I - A A E - L-Leu/Arg
Anabaena sp. 90 McyC - - - C - - - - - - L-Arg
Anabaena sp. 18B6 McyC - - - S - - - - - - L-Arg
Anabaena sp. 66A McyC - - - S - - - - - - L-Arg
Nostoc sp. 152 McyC - - - N - - F I - - L-Arg/Har
Nostoc sp. IO-102-I McyC - - - N - - F - - - L-Arg
Hapalosiphon hibernicus BZ-3-1 McyC - - - - - - - - - - L-Ala
Planktothrix agardhii NIVA126/8 McyC - P - G - - - - - - L-Arg
Planktothrix agardhii 213 McyC - P - C - - - - - - L-Arg
Microcystis aeruginosa PCC 7806 McyC - - - T I - A - - - L-Arg
Microcystis viridis NIES102 McyC - - - I I - A - - - L-Arg
  1. Specificity codes inferred from the protein sequence of the McyB1 and McyC adenylation domains through pairwise alignment against GrsA [20]. The principal amino acid present at the X and Z positions in microcystins analyzed with the LC-MS method.