Skip to main content

Table 1 Sequences of primers used for RACE and RT-PCR analysis

From: Evolutionary conservation of P-selectin glycoprotein ligand-1 primary structure and function

primer name Sequence Ta
GeneRacer™ 5' 5'-CGACTGGAGCACGAGGACACTGA-3' 64°C
Reverse GSP 5'-CAGACCATCTCGGTGGGGGAGTA-3'  
GeneRacer™ 5' Nested 5'-GGACACTGACATGGACTGAAGGAGTA-3' 55°C
Reverse Nested GSP 5'-CACAGTGCACACGAAGAAGATAGTG-3'  
GeneRacer™ 3' 5'GCTGTCAACGATACGCTACGTAACG-3' 55°C
Forward GSP 5'-ACTCCACTGGCAGCCACAGAGG-3'  
GeneRacer™ 3' Nested 5'CGCTACGTAACGGCATGACAGTG-3' 56°C
Forward Nested GSP bovine 5'-CCCTTCCTGTGGCCTCTGATACTC-3'  
Forward Nested GSP pig 5'-ACCAGCACCCACGGAGGCACAGACC-3'  
Forward Nested GSP rat 5'-CCCTGCCAGGGAGTTCAGATCTC-3'  
Forward hPSGL-1/AflII 5'-AGCCTTAAG CCACCATGCCTCTGCAACTCC-3' 54°C
Forward b, pPSGL-1/AflII 5'-TATCTTAAG CCACCATGTTTCTGCAACTCC-3'  
Forward rPSGL-1/AflII 5'-CGCCTTAAG CCACCATGTTCCCACACT-3'  
Forward ePSGL-1/AflII 5'-AGCCTTAAG CCACCATGCCTCTGCCGCTC-3'  
Reverse h, b, p, r, ePSGL-1/AgeI/ClaI 5'-TGGACCGGT ATCGAT AGGGAGGAAGCTGTG-3'  
Forward h, b, p, rFucT-VII 5'-TCCTTGTCTGGCACTGG-3' 50°C
Reverse h, b, p, rFucT-VII 5'-GCGGTGCTGGGAGTTCT-3'  
Forward h, b, p, rβ-actin 5'-GAGACCTTCAACACCCC-3' 50°C
Reverse h, b, p, rβ-actin 5'-GTGGTGGTGAAGCTGTAGCC-3'  
  1. Ta, annealing temperature; GSP, gene specific primer; h, human; b, bovine; p, pig; r, rat; e, equine; FucT-VII, α1–3 fucosyltranferase-VII.