L- and P-selectin-dependent recruitment of neutrophils is species-dependent. Human, bovine, porcine, rat and equine neutrophils were perfused under 0.5–2.0 dynes/cm2 on human P-selectin/μ or on human L-selectin/μ chimera adsorbed on coverslips precoated with goat anti-human IgM antibody. PSGL-1-dependent neutrophil rolling was abolished in presence of 10 mM EDTA. Cell recruitment was assessed at 4–5 min of perfusion. Results represent the mean ± SEM of 3–4 experiments (*, P < 0.05, ***, P < 0.001).