Figure 5
From: Molecular evolution of type VI intermediate filament proteins
Reactivity of the HR-specific monoclonal antibody VAP-5 with the "hypothetical" mouse protein HRM. A fragment of mouse genomic clone RP2389A3 (gi: 106520665) showing the highest sequence identity to chick transitin HR domain has been subcloned in a pET-based expression vector and a His-tag-HRM fusion protein bacterially produced under IPTG induction. A co-migrating immunoreactive band was observed in Western blots using the anti-His-tag antibody and VAP-5 in extracts of bacteria submitted to IPTG induction but was not detected in the uninduced culture.