Figure 4

In vitro ATPase and GTPase activity of the HR domain. The HR domain of chicken transitin and the CTR domain of mouse nestin were expressed in bacteria and purified. Their ATPase/GTPase activities were tested individually using the malachite green colorimetric method for phosphate release determination. Phosphate release was determined at 25°C for each recombinant protein incubated with either A) ATP or B) GTP. The optical density at 650 nm was determined in triplicate at regularly timed intervals during the course of the enzymatic reaction and the mean values are presented with standard deviation error bars. Control: Buffer with the HR recombinant protein without NTP.