Phylogeography of the Solanaceae-infecting Basidiomycota fungus Rhizoctonia solani AG-3 based on sequence analysis of two nuclear DNA loci

Table 1 Descriptive analysis of molecular variation within two cloned nuclear DNA fragments from samples of Rhizoctonia solani AG-3 isolates from potato and tobacco

Sample of isolates	Geographical origin	Total number of mutations, Eta	Number of polymorphic (segregating) sites	Number of haplotypes, NHap	Haplotype (gene) diversity ± standard deviation	Nucleotide diversity, Pi	Average number of nucleotide differences, k	Sampling variance of k, Vs(k) ^b	Total variance of k, V(k) ^b
Locus ^a	pP42F ^a
Total sample: potato and tobacco		48	45	16	0.856 ± 0.048	0.03769	15.113	0.265	5.303
Potato	Eastern NC, Maine and Wisconsin	22	20	14	0.942 ± 0.026	0.01340	5.373	0.156	1.947
Tobacco	Central NC and Southern Brazil	11	11	2^b	0.133 ± 0.112	0.00366	1.467	0.070	0.559
Locus ^a	pP89 ^a
Total Sample: potato and tobacco		100	98	22	0.869 ± 0.048	0.03486	37.862	0.647	13.268
Potato	Eastern NC, Maine and Wisconsin	32	32	20	0.963 ± 0.029	0.00703	7.640	0.212	2.759
Tobacco	Central NC and Southern Brazil	16	16	2^b	0.133 ± 0.112	0.00196	2.133	0.102	0.813

^a The total number of sites analyzed (excluding sites with alignment gaps) was 401 (pP42F) and 1090 (pP89).
^b The variances of k were estimated assuming free recombination.
^c One single allele was detected in the North Carolina sample of tobacco isolates.

ISSN: 2730-7182