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Table 2 Results of culture experiments: ability of clonal cercomonad strains to grow in various marine media and other strain characteristics

From: DNA evidence for global dispersal and probable endemicity of protozoa

Cladea

Strain

18S identityb

ITS1 identityb

Morph. identityc

Salinity toleranced

Size (μm)e

Cystsf

Vigourg

α

XT179

1

1

1

() (motile cells)

5–7

α

NZ1.5C

1

1

1

5–6

α

XT160

1

1

1

5–7

α

EC

1 *

2 *

1

(immobile cells)

5–7

X?

α

5A

1

2 *

2

N/A

6–8

X?

α

P111

1

3

N/A

N/A

5–7

X

α

SCCAP C1h

1

N/A

N/A

N/A

4–10h

()h

N/A

γ

C73

1

1

N/A

4–6

X

γ

XT196

1

2

N/A

N/A

5–10

N/A

γ

C76

2

3

N/A

X

5

X

γ

11.7E

3

4

N/A

5–7

  1. N/A = no data
  2. * Although the ITS1 sequences for EC and 5A are identical, and 18S rDNA is identical in the V4 region (on which Fig. 1 is based), EC has an insert near the 3' end of 18S and is therefore clearly a different strain. This is the only strain in this study known to have such an insert.
  3. a see Figs 1, 2, and 3.
  4. b Identical numbers indicate strains with identical sequences.
  5. c Identical numbers indicate strains with strain traits other than size (flagella length:body length, form and frequency of plasmodium formation, propensity to form cell clusters, etc.) which were indistinguishable from others in the same medium under light microscopy (200× phase contrast).
  6. d Overall performance of strains under a range of saline medium regimens, as described in Methods. X means that no living cells were seen.
  7. e Size range determined from 3+ independent cultures observed over a period of 18 months.
  8. f Indicates the propensity of the strain to form cysts in Volvic & grain cultures, summarized from observations of 3+ independent cultures observed over a period of 18 months. X = no cysts seen, = cysts infrequent and sparse, = cysts regular at low to medium densities, = cysts readily formed and seen at high densities.
  9. g A measure of the ability of the strain to form dense cultures in Volvic & grain medium, which persist well over time (2+ months in unmanipulated cultures), summarized from observations of 3+ independent cultures observed over a period of 18 months. = rarely or never reach high density, = may reach medium to high density but do not persist, = usually reach high densities and/or persist for long periods without encysting or disappearing from the culture.
  10. h See ref. 31. Not included on Figs 1 and 3 as there is no ITS1 sequence available. Not included in culture experiments.
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BMC Ecology and Evolution

ISSN: 2730-7182

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