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Figure 3 | BMC Evolutionary Biology

Figure 3

From: DNA evidence for global dispersal and probable endemicity of protozoa

Figure 3

Global provenance, biogeography and phylogeny of cercomonads. (A) World map showing major animal biogeographical regions. (B-D) 18S-ITS1 rDNA phylogenies of groups α (278 nucleotide positions), β (414 positions), and γ (335 positions). See Methods for details of each sequence dataset. Coloured discs correspond to hoops on map. Grey arrowheads indicate sequences found in only one biogeographical region. Square black brackets indicate 18S-genotype boundaries. X and Z (bracketed) indicates a possible endemic genotypic radiation. Branches marked a, f, and h in (B) are discussed in relation to additional sampling and ITS-type-specific primer probing. Branches without coloured discs originate from cultures. Sampling range differs among the three groups (Table 1). Key to bootstraps (ML/parsimony) given to right of scale. Bootstraps shown only when equal to or exceeding the value stated, although precise values are shown for clades marked X and Z, as these are discussed in the text as potentially geographically-restricted clades. Branch labels underlined and in bold refer to culture-derived sequences, including those used in the culture experiments. NB: in some cases a single branch corresponds to >1 culture or both environmental and culture-derived sequences. The long branch for 11.7E is not shown in (D) for the sake of clarity. It was not detected in any environmental library.

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