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Figure 6 | BMC Evolutionary Biology

Figure 6

From: Dynamic expression of ancient and novel molluscan shell genes during ecological transitions

Figure 6

Sequence and expression analysis of Has-tsfgr1. (A) A schematic representation of the conceptually derived Has-tsfgr1 protein. A putative signal peptide followed by 15 residues constitute the amino end of the protein. 11 glycine rich domains then follow, predominantly containing the motif 'GGYGLGL'. Boxes of the same colour indicate identical sequence motifs. 5 residues at the carboxyl terminus (GYKGY) may share functional homology with the Shematrin proteins. (B) The Has-tsfgr1 transcript is 642 bp long and contains a polyadenylation signal (bold). Primers used to assess temporal expression are boxed, and the predicted signal sequence cleavage site is indicate by ↑. (C) An alignment of the repetitive domains of Has-tsfgr1 reveal the high degree of sequence conservation between motifs. (D) Has-tsfgr1 transcripts are initially detected in trochophore larvae within the expanding shell field (sf) with relatively strong expression in an anterior pair of cells (arrows). (E) Early post-torsional veligers express Has-tsfgr1 in a triplet of cells spread along the developing mantle edge on the left side of the animal. This lateral view presents the animal with anterior to the left. (F) Competent veligers express Has-tsfgr1 continuously along the mantle edge. The eyespot (es), radula (r) and refractive operculum (op) are indicated. (G) Intense expression of Has-tsfgr1 is detected within the mantle of 1 mm juveniles. (H) A magnified view of the boxed region in G reveals that this expression is restricted to the anterior edge of the outer mantle fold. The apical expression of Has-tsfgr1 within these cells is clearly visible (nuclei indicated by arrowheads).

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