Figure 1

Fluorescence in situ hybridization of tiger beetle chromosomes with a ribosomal probe. A. Cicindela punctulata, n = 9 + XXXY, early prophase I nucleus showing two rDNA loci, one located on the Y and another located on one of the four X chromosomes. B. C. scutellaris, diakinesis with n = 9 + XXXY showing two rDNA loci located on an autosomal pair. C. C. repanda, diakinesis with n = 9 + XXXY showing two rDNA loci located on an autosomal pair D. C. tranquebarica, diakinesis with n = 9 + XXXY showing three rDNA loci, two located on an autosomal pair and one located on one of the 3 X chromosomes. E. C. ocellata, metaphase I with n = 9 + XXY showing two rDNA loci located on an autosomal pair and one on the X chromosome. F. C. marginata, spermatogonial metaphase with 2n = 23 chromosomes showing six rDNA loci, one located on each of the four X chromosomes and two loci located on the Y chromosome. The white arrows indicate the heterosomal loci, the empty arrows indicate the autosomal loci. The arrowheads point to the sex chromosomes which are condensed in Fig. 1D forming the sex vesicle, and are recognizable individually forming the rosette-like structure in Figs. 1A, B, C, and E.