Translational machinery of the chaetognath Spadella cephaloptera: a transcriptomic approach to the analysis of cytosolic ribosomal protein genes and their expression

Table 4 Presence of members of 4 ribosomal gene families in three S. cephaloptera individuals using PCR

			ESTs with TTT sites					ESTs with TAC sites
RP	Is.	Name of the reverse primers (5'-3')	EMBL acc. n° of ESTs which bear the primer sequences	EMBL acc. n° of ESTs with 1 or 2 internal mutations in the primer sequences	PCR results by individual			EMBL acc. n° of ESTs which bear the primer sequences	PCR results by individual
					1	2	3		1	2	3
S8	1	S8-1R	CR952841, CR953486	CR952238, CR953840	+	+	+	CR952310, CR953241	+	+	+
	2	S8-2R	CR952852, CR953783	none	+	+	+	CR952219, CR953331, CR952138, CR952189	+	+	+
	3	S8-3R	CR952624, CR952678	none	-	-	-	none	-	-	-
S25	1	S25-1R	CR952125, CR952226, CR952356, CR952457, CR952866, CR953401, CR953717, CR953720, CR954024, CR954106	CR952061, CR952409, CR953636	+	+	+	CR953753	+	+	+
	2	S25-2R	CR954093, CR953826	none	+	+	-	none	-	-	-
	3	S25-3R	none	none	-	-	-	CR953802	-	-	+
L15	1	L15-1R	CR953910	none	+	+	-	none	+	-	+
	2	L15-2R	CR952649	none	+	-	+	none	-	-	+
L27a	1	L27a-1R	CR952520, CR952575, CR953281, CR953937	CR952087, CR952954, CR953676	+	+	+	CR953609	+	+	+
	2	L27a-2R	none	CR952757	+	+	+	CR952635, CR953421	+	+	+

For all the members of these four gene families, no internal mutations in the regions bearing the putative TAC sites have been found. Abbreviations and symbols: none, no EST clone has been found in the S. cephaloptera library; RP, ribosomal protein; is., isoform; Acc. n°, EMBL accession numbers; +, PCR with positive results; -, PCR with negative results.

ISSN: 2730-7182