Figure 14

The phylogenetic tree derived from 54 G-protein beta AA sequences. (A) illustrates the upper part of the tree and (B) does the lower part. a and a, and b and b' indicate the continuation of branches from the upper part to the lower. A total of 54 AA sequences of eukaryotic G-protein beta and two beta-like AA sequences were analyzed [see Additional file 8]. The outgroup was 7 bacterial WD-repeat protein AA sequences. Tunicate GNBs were underlined. AA sequences are inferred from the established genome data bases, JGI, NCBI genomes, and Ensembl. Alignment was carried out by the ClustalX 1.83 program. The gaps within nonpreserved regions were carefully deleted manually by the BioEdit program. The phylogenetic tree derived from the aligned data was then made by the Neighbor Joining Method in the Mega3 v3.1 program, using the Amino Poisson correction model with Gamma-distributed Rates among sites (Gamma parameter 2.0) and Bootstrap as the test of inferred branches (Repetition 500), including 471 sites with a pairwise deletion of Gaps/Missing Data. Drosophila and Anopheles Gbeta76C genes may be differently grouped from the vertebrate-type GNBs according to this phylogenetic tree. Those bacterial AA sequences of WD-repeat proteins were also referred from the NCBI microbial genome database. Abbreviations: GNB, G-protein beta. Rosfsp, Roseiflexus sp.; Anavar, Anabaena variabilis; Nossp, Nostoc sp.; Nospc, Nostoc punctiforme; Glovio, Gloeobacter violaceus.