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Table 2 List of variables used in statistical analyses

From: Evolution of dispersal and life history strategies – Tetrahymena ciliates

Experiment

Variable name

Definition

Estimation method and level

Growth

K

Carrying capacity (see eqn 1)

Logistic function fitted at the replicate level to successive densities (time > 0) estimated at the picture level (5 values per time step)

 

r

Growth rate (see eqn 1)

Logistic function fitted at the replicate level to successive densities (time > 0) estimated at the picture level (5 values per time step)

 

Final cell density (at K)

Mean density of cells at the last time step (192 hours)

Mean of 5 pictures per replicate

 

Final cell size (at K)

Mean size of cells at the last time step (192 hours)

Mean of all cells of the 5 pictures per replicate

 

Final cell shape (at K)

Mean shape of cells at the last time step (192 hours)

Mean of all cells of the 5 pictures per replicate

Starvation

Survival as a density sum over time

Area between the density curve and the density at time = 0 line, starting at time = 8 h which was the mean peak density for all strains

Curves defined by successive values through time of the mean of the 5 pictures per replicate

 

Mean maximal cell elongation

Difference between longest shape and start shape, expressed in percentages of start shape

Estimated on means of all cells in the 5 pictures per replicate

 

Variance in maximal cell elongation

Difference between the 95th upper percentile and the mean of the shape distribution at the time when the maximal elongation is reached, expressed as percentage of the same difference at start

Estimated on means of all cells in the 5 pictures per replicate

 

Elongation persistence

Sum of elongation over time, i.e. area between the shape curve and the shape at time = 0 line, starting at time = 8 h

Curves defined by successive values through time of the mean of the 5 pictures per replicate

 

Frequency of putative disperser morphs

Frequency of disperser morphs observed at time = 8 h

Mean of the 5 values per replicate (recorded on each sample just after picture was taken)

Dispersal

Initial cell shape

Mean shape of cells in the initial tube

Mean of all cells of the 10 pictures per block

 

Dispersal rate

Proportion of cells in the target tube at the end of the experiment

Computed as a combination of parameter values at the replicate level

 

Cell elongation

Difference between mean cell shape in target tube and start tube, expressed as percentage of the shape in start tube 1

Computed as a combination of parameter values at the replicate level

Colonization

Colonization probability

Proportion of tubes where a cell population developed from the single cell added to the tube

 
  1. 1 Estimating cell elongation relative to initial shape gave nearly identical results (results not shown).