Figure 3

Changes in aging after 2000 generations of evolution. Population 1 (top panel) and population 3 (bottom panel) showed a slower decline in the reproductive function with age than the ancestor (grey line). The difference to the ancestor is significant (at p < 0.001) for both populations. Population 2 (middle panel) shows variation in the decline of the reproductive function with age. From each population, 30 or more cells from 5 independent clones were assayed, and each line represents the moving average of measurements from one clone, standardized to an intercept of 1. Statistic analysis was performed on the non-transformed data with logistic regression.