Figure 2

Trs120 is conserved in almost all eukaryotes. A. The domain structure of Trs120 is shown. Hs and Sc show amino acid coordinates in H. sapiens and S. cerivisiae, respectively. HC indicates highly-conserved amino acids described in Table S2. "Sizes" shows the median amino-acid lengths of all inter- and intra-domain lengths (intra-domain sizes are in bold). Arrows indicate a functional breakpoint. C-terminal truncation from this point results in a temperature-sensitive growth phenotype, while truncation N-terminal to this point is lethal. Actual sequences corresponding to these regions can be viewed at [50]. B. We show the multiple alignment of representative organisms for the sequences of each domain, I-V. Whole sequence alignment of all sequences from the NCBI nr database is shown in Figure S1. Organism abbreviations are as given in Table S1. For each position in the alignment, all amino acids belonging to the popular amino-acid grouping [42] best representative of the characters acids at that position, are marked in grey. Positions without sequence correspond to stretches in the alignment where one or two sequences contain long insertions that do not occur in the other sequences. Boxes were drawn to indicate the HC positions shown in A. To indicate the overall contribution of each of the amino acids at each position, we also show an Alscript [51] diagram above the multiple alignment. The height of each amino acid indicates its relative contribution to the alignment at that position, ignoring insertions (-). Amino acids corresponding to the most common amino acid grouping are written in black, while all other amino acids are written in gray.