Molecular cloning and analysis of zebrafish voltage-gated sodium channel beta subunit genes: implications for the evolution of electrical signaling in vertebrates

Table 5 Primers used to detect expression of zebrafish β subunit genes and splice variants in different tissues of the adult zebrafish.

*Forward Primer (5'-3')*	*Reverse Primer (5'-3')*	*Gene*	*Variant*	*Amplicon Size (bp)*
CTACACTTATGCAGAAATGACAGCCAGC	GATGGACAGAGCTTCAAGCTTTTGGCT	zβ1	A	422
		zβ1	D	494
GACAGAATCCTCATCTTCCCCAACTATG	CTGCATTCTTCATTTAAACTCAGAGGT	zβ1	B	267
		zβ1	D	534
GTCCCTGCGCTGTTGTGTTTAACACAT	GGGTGAACAATCCCTTTAAGCTGCACT	zβ1	C	382
CAGCTGACAGACGAGGGCATCTACAACT	CAACACCTGCAGTGAGAAAACCCCATT	zβ2	A	201
CATCCTTGCTCTGCTCATTCTGTCCAT	TCGCTACACGATAATACCAGGGAGTGT	zβ2	B	304
		zβ2	C	169
		zβ2	D	236
CTGGTGTGTGTGGATGTGCCATCA	CTTGTTGGCGGAAAGCTTGAATGA	zβ3	-	357
AGGTGAGCACAGGGAAGGTCCATT	GGAGGCCATTTTCTGTGTTGTCGT	zβ4	loc1	543
TGTGTTGTGTTCATGCTTTG	GACCACCTTTAGTTCCTCTA	zβ4	loc2	395

Note: several primer pairs detected multiple splice variants, as indicated above and displayed in Figure 5. BP = base pairs or nucleotide number.

ISSN: 2730-7182