Molecular cloning and analysis of zebrafish voltage-gated sodium channel beta subunit genes: implications for the evolution of electrical signaling in vertebrates

Table 3 Comparative genomics of the sodium channel β3 gene in zebrafish and mammals.

gene	Genbank accession number	gene location	cDNA	exon 1	exon 2	exon 3	exon 4	exon 5	exon 6	exon 7	protein	%ID (%SIM)	topology
zβ3	DQ489730	Chr 15	1006	294 (0)	103 (55)	170	235	139	65 (64)	-	220	51.1% (65.2%)	IG(V): 38–150 TM: 165–186
Hsβ3	NM_018400	Chr 11	4052	778 (0)	80 (55)	164	226	139	86 (64)	2579 (0)	215	100% (100%)	IG(V): 38–145 TM: 160–181
Mmβ3	NM_178227	Chr 9	3549	206 (0)	80 (55)	164	226	139	80 (64)	2654 (0)	215	97.7% (97.7%)	IG(V): 38–145 TM: 160–181
Rnβ3	NM_139097	Chr 8	3910	350 (0)	82 (55)	164	226	139	80 (64)	2837 (0)	215	98.1% (98.1%)	IG(V): 38–145 TM: 160–181

Presentation and labeling as in Table 1. %ID (%SIM) refers to percentage identity and similarity with the human β3 protein sequence as determined by alignment. Zebrafish β3 subunit topology was determined as described in Methods. Numbering refers to β3 protein sequence with signal peptide intact. IG(V) domain and transmembrane regions for human β3 and human/mouse/rat splice variants were annotated based on previous reports [12, 14].

ISSN: 2730-7182