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Table 1 Primers used for real-time PCR gene expression analysis. F and R refer to forward and reverse primers, respectively.

From: Comparative sequence analysis of the complete set of 40S ribosomal proteins in the Senegalese sole (Solea senegalensis Kaup) and Atlantic halibut (Hippoglossus hippoglossus L.) (Teleostei: Pleuronectiformes): phylogeny and tissue- and development-specific expression

Target Primers    Fragment size (bp)
  Primer pair name Sequence 5'-position  
RPS2 SserpS2•1 5'-CCAAGCTGTCGATTGTCCCGGTCA-3' (F) 434 127
  SserpS2•2 5'-CGGGGGGCAGGGATGAGACG-3' (R) 560  
RPS3a SserpS3a•1 5'-TCAGAAAGACCTCCTACGCCCAGCA-3' (F) 450 94
  SserpS3a•2 5'-AGATCATTGGTCTGAACCTCACGGGTCA-3' (R) 543  
RPS15 SserpS15•1 5'-CATGGTTGGCGTGTACAATGGCAAA-3' (F) 300 116
  SserpS15•2 5'-GGCGACCGTGCTTGACTGGCTTG-3'(R) 415  
RPS27-1 SserpS27-1•1 5'-CCCGAGGAGGAGAAGAGGAGGCACA-3' (F) 64 124
  SserpS27-1•2 5'-CTGTCTGAGCGTGACTGAACACCGTCGT-3' (R) 187  
RPS27-2 SserpS27-2•1 5'-GCTAAAGACCTCCTCCACCCTGCCATT-3' (F) 72 130
  SserpS27-2•2 5'-ACACAGTTGTGATTTTGTAGCAGCCTGGAC-3' (R) 201  
RPS27a SserpS27a•1 5'-GCGTGAGTGTCCGGCTGACGA-3' (F) 431 87
  SserpS27a•2 5'-GTGAGGCAGCACTTCCCGCAGT-3' (R) 517  
RPS28 SserpS28•1 5'-CGATAGTTCCCGCTGAAGCTGTGAGGTG-3' (F) 206 95
  SserpS28•2 5'-GAGAATGTGAGGGATGTCCGCCGTTG-3' (R) 300  
RPS29 SserpS29•1 5'-AGGCAGTACGCTAAAGACATCGGCTTCGTG-3' (F) 135 121
  SserpS29•2 5'-GTGCTGAATTATCCCATCATCTTGGCTGGT-3' (R) 255  
GAPDH SseGAPDH231•1 5'-AGCCACCGTGTCGCCGACCT-3' (F) 1001 107
  SseGAPDH231•2 5'-AAAAGAGGAGATGGTGGGGGGTGGT-3' (R) 1107  
Ubiquitin SseUB•1 5'-AGCTGGCCCAGAAATATAACTGCGACA-3' (F) 289 93
  SseUB•2 5'-ACTTCTTCTTGCGGCAGTTGACAGCAC-3' (R) 381