(A) Map of collection sites in Australasia with proportional distribution of Cx. annulirostris COI lineages. Collection sites are indicated 1–30 (for locality details see Table 1, Supplementary see Additional file 1 and 2). Pie chart graphs indicate the distributional frequency of Cx. annulirostris haplotypes representing the five identified mtDNA COI lineages. The size of the pie charts' segments is proportional to the number of mosquitoes identified as Cx. annulirostris, which is also indicated in brackets. Haplotype a51 from the laboratory colony at AMI in Queensland, Australia, is not included. (B) Summarized Bayesian phylogenetic tree. Phylogenetic tree of Cx. annulirostris showing 134 COI haplotypes compressed into the 5 different lineages of Cx. annulirostris. Branch support is Bayesian posterior probability/ML bootstraps (expanded in Figure 2). Haplotypes belonging to Cx. sitiens were used as an outgroup.