Figure 1

SmTRC1 elements. A: Agarose gel electrophoresis of typical PCR amplification products of S. mansoni genomic DNA with primers designed from the sequence of SmTRC1 extremities. B: Schematic representation of the SmTRC1 element derived in silico from S. mansoni shotgun genomic sequencing and assembly data obtained from the Sanger Institute (Supercontig 0018735) or from direct sequencing of clones amplified by PCR from genomic DNA obtained in this work (SmTRC1f1 and SmTRC1d1). Black boxes indicate the Terminal Inverted Repeats (TIR). Light gray boxes indicate the predicted SmTRC1-ORF and the dark gray box indicates the Transposase_21 domain within this ORF. The hatched box indicates a region with tandem repeats.