Figure 4

Phylogenetic tree based on an alignment of PRP8 proteins. The tree was constructed by the neighbour-joining method using PAUP* [61] and is a consensus derived from 1000 bootstrap replicates. The numbers indicate the percentage bootstrap support (only nodes with >50% support are shown). Accession numbers for the PRP8 sequences are: Caenorhabditis elegans, NM_066384; Mus musculus, AB047391; Homo sapiens, BAA22563; Xenopus laevis, AAH45266; Anopheles gambiae, EAA04255; Drosophila melanogaster, NP610735; Oryza sativa, AB023482; Arabidopsis thaliana, AC009322; Schizosaccharomyces pombe, T38841; Neurospora crassa, EAA33717; Aspergillus nidulans, BK001316; Saccharomyces cerevisiae, S34670; S. paradoxus, AABY01000045 (ORF: 35077-42312); S. mikatae, AABZ01000010 (ORF: 37550-44800); Neurospora crassa (EAA33717); Dictyostelium discoideum, AAL92617. PRP8 sequences of Candida glabrata (gene name CAGL0I02266g), Debaryomyces hansenii (gene name DEHA0E02717g) and Kluyveromyces lactis (gene name KLLA0A05280g) were obtained from the Génolevures website. Predicted PRP8 protein sequences for the remaining species were generated using data from their respective genome sequencing web sites and are available from the authors. Accession numbers refer to data which include the PRP8 encoding regions; Cryptococcus neoformans var. grubii (AACO01000004), Cryptococcus neoformans var.neoformans (AAEY01000001), Cryptococcus gattii R265 (AAFP01000516), Coprinopsis cinerea (AACS01000175), Gibberella zeae (AACM01000127), Ashbya gossypii (AAS50980), Aspergillus fumigatus (AAHF01000008), Aspergillus clavatus (AAKD01000005), Aspergillus terreus (AAJN01000238), Neosartorya fischeri (AAKE01000005.1), Uncinocarpus reesii (AAIW01000130.1), Coccidioides immitis (AAEC02000024), Trichoderma reesei (AAIL01001072), Magnaporthe grisea (AACU02000289), Chaetomium globosum (AAFU01000976), Phaeosphaeria nodorum (AAGI01000010), Ustilago maydis (AACP01000056), Phanerochaete chrysosporium (AADS01000435), Botrytis cinerea (AAID01001534, AAID01001533), Sclerotinia sclerotiorum (AAGT01000110), Rhizopus oryzae (AACW02000011), and Histoplasma capsulatum (AAJI01001309).