Figure 1

A, The oligomerization domains of fish and representative tetrapod TSPs. Oligomerization regions identified by the COILS program were aligned by the program TCOFFEE. The positions of the two adjacent conserved cysteine residues are indicated with arrows (subgroup A) or asterisk (subgroup B). B, Alignment of the sixth type 3 repeat of vertebrate subgroup B TSPs, displaying the PPGP motif that is absent from mammalian TSP-5s. Each alignment is presented in Boxshade 3.2. Black shading indicates identical amino acids, grey shading indicates conservative substitutions and white background indicates unrelated amino acids. Key : Dr = Danio rerio; Gg = Gallus gallus; Hs = Homo sapiens; Mm = Mus musculus; Tn = Tetraodon nigriviridis; Tr = Takifugu rubripes; Xt = Xenopus tropicalis.