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Table 1 List of degenerate primers and primers used for the reverse transcription PCR (RT-PCR), rapid amplification of cDNA ends (RACE) and Real-time PCR. The black vertical bar next to the primer names indicates which combinations of primers were used together.

From: Evolution of the P-type II ATPase gene family in the fungi and presence of structural genomic changes among isolates of Glomus intraradices

Degenerate primers sense 5'-3' sequence
ATP.Deg1 Forward TGY WSY GAY AAR ACY GGI AC
ATP.Deg2 Reverse TTV ACH CCR TCH CCI GTC AT
ATP.Deg3 Reverse ATN SWN GCR AAR TTR TCR TC
ATP.Deg4 Forward TGY WSN GAY AAK ACN GG
ATP.Deg5 Reverse ACN CCR TCN CCN GTC AT
ATP.deg6 Forward NGC RAA RTT RTC RTC CAT
ATP.Deg7 Reverse AAR TTR TCR TCC ATN ARD AT
ATP.Deg8 Reverse TAR RTN RTN CCN GCN GGN
RT-PCR sense 5'-3' sequence
SERCAGlo.F Forward ACA ACG AAC CGT ATG AGC GT
SERCAGlo.R Reverse GCA AGA ACC ATA TCA GCA GC
PMCAGlo.F Forward ACC CAA AAC AGA ATG ACC
PMCAGlo.R Reverse ATC GAA GAA GCT TCC TTA GC
NaKGlo.F Forward TTA ACT CGA AAT CAA ATG AC
NaKGlo.R Reverse CGT GCA AAA ATA ATT TCA TC
ENAGlo.F Forward ACA CTA ACA CAG AGT AAA ATG
ENAGlo.R Reverse AAT CAT TTT TAC TTT TGT ATC
RACE sense 5'-3' sequence
SERCA.GSP1 5' RACE CGC TCA TAC GGT TCG TTG TAA GCG T
SERCA.GSP2 3' RACE TAT AGC AAT GGG TGA CGG TAC CGA T
PMCA.GSP1 5' RACE CAC CAG TAG CAG GTG CTT TTC CGT G
PMCA.GSP2 3' RACE CTT GCT CGT TCT TCT CCA ACC GAT A
NK.GSP1 5' RACE AGC ATC ACC AAG AAT TGC ACG TTC A
NK.GSP2 3' RACE ACT GGT CGA CCT ATT GAG TCA ATT C
ENA.GSP1 5' RACE GAA GCC AAG CAT CAG TTG CAA TCA T
ENA.GSP2 3' RACE TGA CTG CCG CAC AGT TTG ATG CAC T
Real-time PCR sense 5'-3' sequence
ENA.real.F1 Forward AAC TTG CAA GCA AAG GGA TG
ENA.probe 1 Probe TTG GCA GCA TAC CGT CGA GTT
ENA.real.R1 Reverse GTG GAT CAT AAA TAC CAA CCA
ENA.real.F2 Forward CAA GAA TAT GCG TTT GAC ACT GAA
ENA.probe 2 Probe TGA AAC GTA TGT CTG TTG TAT GTA AAG AAA AAT CTA CGG A
ENA.real.R2 Reverse AAA CAG ATT CTG TTG CGC CTT TA