Evolution of the P-type II ATPase gene family in the fungi and presence of structural genomic changes among isolates of Glomus intraradices

Table 1 List of degenerate primers and primers used for the reverse transcription PCR (RT-PCR), rapid amplification of cDNA ends (RACE) and Real-time PCR. The black vertical bar next to the primer names indicates which combinations of primers were used together.

Degenerate primers	sense	5'-3' sequence
ATP.Deg1	Forward	TGY WSY GAY AAR ACY GGI AC
ATP.Deg2	Reverse	TTV ACH CCR TCH CCI GTC AT
ATP.Deg3	Reverse	ATN SWN GCR AAR TTR TCR TC
ATP.Deg4	Forward	TGY WSN GAY AAK ACN GG
ATP.Deg5	Reverse	ACN CCR TCN CCN GTC AT
ATP.deg6	Forward	NGC RAA RTT RTC RTC CAT
ATP.Deg7	Reverse	AAR TTR TCR TCC ATN ARD AT
ATP.Deg8	Reverse	TAR RTN RTN CCN GCN GGN
RT-PCR	sense	5'-3' sequence
SERCAGlo.F	Forward	ACA ACG AAC CGT ATG AGC GT
SERCAGlo.R	Reverse	GCA AGA ACC ATA TCA GCA GC
PMCAGlo.F	Forward	ACC CAA AAC AGA ATG ACC
PMCAGlo.R	Reverse	ATC GAA GAA GCT TCC TTA GC
NaKGlo.F	Forward	TTA ACT CGA AAT CAA ATG AC
NaKGlo.R	Reverse	CGT GCA AAA ATA ATT TCA TC
ENAGlo.F	Forward	ACA CTA ACA CAG AGT AAA ATG
ENAGlo.R	Reverse	AAT CAT TTT TAC TTT TGT ATC
RACE	sense	5'-3' sequence
SERCA.GSP1	5' RACE	CGC TCA TAC GGT TCG TTG TAA GCG T
SERCA.GSP2	3' RACE	TAT AGC AAT GGG TGA CGG TAC CGA T
PMCA.GSP1	5' RACE	CAC CAG TAG CAG GTG CTT TTC CGT G
PMCA.GSP2	3' RACE	CTT GCT CGT TCT TCT CCA ACC GAT A
NK.GSP1	5' RACE	AGC ATC ACC AAG AAT TGC ACG TTC A
NK.GSP2	3' RACE	ACT GGT CGA CCT ATT GAG TCA ATT C
ENA.GSP1	5' RACE	GAA GCC AAG CAT CAG TTG CAA TCA T
ENA.GSP2	3' RACE	TGA CTG CCG CAC AGT TTG ATG CAC T
Real-time PCR	sense	5'-3' sequence
ENA.real.F1	Forward	AAC TTG CAA GCA AAG GGA TG
ENA.probe 1	Probe	TTG GCA GCA TAC CGT CGA GTT
ENA.real.R1	Reverse	GTG GAT CAT AAA TAC CAA CCA
ENA.real.F2	Forward	CAA GAA TAT GCG TTT GAC ACT GAA
ENA.probe 2	Probe	TGA AAC GTA TGT CTG TTG TAT GTA AAG AAA AAT CTA CGG A
ENA.real.R2	Reverse	AAA CAG ATT CTG TTG CGC CTT TA

ISSN: 2730-7182