Table 1 List of degenerate primers and primers used for the reverse transcription PCR (RT-PCR), rapid amplification of cDNA ends (RACE) and Real-time PCR. The black vertical bar next to the primer names indicates which combinations of primers were used together.
Degenerate primers | sense | 5'-3' sequence |
|---|---|---|
ATP.Deg1 | Forward | TGY WSY GAY AAR ACY GGI AC |
ATP.Deg2 | Reverse | TTV ACH CCR TCH CCI GTC AT |
ATP.Deg3 | Reverse | ATN SWN GCR AAR TTR TCR TC |
ATP.Deg4 | Forward | TGY WSN GAY AAK ACN GG |
ATP.Deg5 | Reverse | ACN CCR TCN CCN GTC AT |
ATP.deg6 | Forward | NGC RAA RTT RTC RTC CAT |
ATP.Deg7 | Reverse | AAR TTR TCR TCC ATN ARD AT |
ATP.Deg8 | Reverse | TAR RTN RTN CCN GCN GGN |
RT-PCR | sense | 5'-3' sequence |
SERCAGlo.F | Forward | ACA ACG AAC CGT ATG AGC GT |
SERCAGlo.R | Reverse | GCA AGA ACC ATA TCA GCA GC |
PMCAGlo.F | Forward | ACC CAA AAC AGA ATG ACC |
PMCAGlo.R | Reverse | ATC GAA GAA GCT TCC TTA GC |
NaKGlo.F | Forward | TTA ACT CGA AAT CAA ATG AC |
NaKGlo.R | Reverse | CGT GCA AAA ATA ATT TCA TC |
ENAGlo.F | Forward | ACA CTA ACA CAG AGT AAA ATG |
ENAGlo.R | Reverse | AAT CAT TTT TAC TTT TGT ATC |
RACE | sense | 5'-3' sequence |
SERCA.GSP1 | 5' RACE | CGC TCA TAC GGT TCG TTG TAA GCG T |
SERCA.GSP2 | 3' RACE | TAT AGC AAT GGG TGA CGG TAC CGA T |
PMCA.GSP1 | 5' RACE | CAC CAG TAG CAG GTG CTT TTC CGT G |
PMCA.GSP2 | 3' RACE | CTT GCT CGT TCT TCT CCA ACC GAT A |
NK.GSP1 | 5' RACE | AGC ATC ACC AAG AAT TGC ACG TTC A |
NK.GSP2 | 3' RACE | ACT GGT CGA CCT ATT GAG TCA ATT C |
ENA.GSP1 | 5' RACE | GAA GCC AAG CAT CAG TTG CAA TCA T |
ENA.GSP2 | 3' RACE | TGA CTG CCG CAC AGT TTG ATG CAC T |
Real-time PCR | sense | 5'-3' sequence |
ENA.real.F1 | Forward | AAC TTG CAA GCA AAG GGA TG |
ENA.probe 1 | Probe | TTG GCA GCA TAC CGT CGA GTT |
ENA.real.R1 | Reverse | GTG GAT CAT AAA TAC CAA CCA |
ENA.real.F2 | Forward | CAA GAA TAT GCG TTT GAC ACT GAA |
ENA.probe 2 | Probe | TGA AAC GTA TGT CTG TTG TAT GTA AAG AAA AAT CTA CGG A |
ENA.real.R2 | Reverse | AAA CAG ATT CTG TTG CGC CTT TA |