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Table 3 Summary of RT-PCR analysis of island 4305 and adjacent gene expression in a range of Gram negative hosts.

From: A new experimental approach for studying bacterial genomic island evolution identifies island genes with bacterial host-specific expression patterns

  

STM gene

  

4302

4304

4305

4309

4310

4315

4316

4318

4319

4320

trfA

inter*

 

Salmonella typhimurium

++

++

++

+

++

++

++

++

++

++

++

-

 

S. typhimurium island 4305 (R995 + island 4305)

++

++

++

+

++

++

++

++

++

++

++

-

Gamma proteobacterial hosts for (R995 + island 4305)

Escherichia coli

++

++

++

++

++

+

++

++

++

++

++

-

 

Pseudomonas putida

++

++

++

++

++

++

++

++

++

+

++

-

 

Pseudomonas aeruginosa

++

++

++

++

+

++

++

++

+

++

++

-

 

Vibrio vulnificus

++

++

++

++

++

+

++

++

++

++

++

-

 

Pseudomonas stutzeri

++

++

++

++

++

++

++

++

+

++

++

-

Alpha proteobacterial hosts for (R995 + island 4305)

Sphingomonas paucimobilis

++

++

++

++

++

++

++

++

++

++

++

-

 

Novosphingobium capsulatum

++

++

++

-

-

+

++

++

+

++

++

-

 

Agrobacterium tumefaciens

++

++

++

++

-

-

-

++

++

++

++

-

 

Rhizobium meliloti

++

++

++

++

-

-

++

++

-

++

++

-

 

Rhodobacter sphaeroides

++

++

++

-

-

-

+

++

-

++

++

-

  1. "++" is an expression ratio of 1.0 – 0.2 compared to trfA (easily detectable) as in Materials and Methods; " +" is a ratio of 0.2 -0.02 (barely detectable); " -"is a ratio of ≤0.02 (i.e. non-detectable)
  2. * Intergenic region predicted to be non-transcribed between STM4304 and 4305.