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Figure 2 | BMC Evolutionary Biology

Figure 2

From: A new experimental approach for studying bacterial genomic island evolution identifies island genes with bacterial host-specific expression patterns

Figure 2

Characterization of the IS200 region of island 4305. Panel A depicts the genes STM4310, 4311, and 4312 in S. typhimurium island 4305. Gene STM4311 is the IS200 transposase that is present in strain LT2. Small arrows indicate primers that were used to amplify the indicated region between STM4310 and 4312 in strains LT2, χ3339, and UK-1. The DNA agarose gel displays the products from PCR reactions using the indicated primers to amplify chromosomal DNA isolated from the following hosts: lane 1, LT2; lane 2, χ3339; lane 3, UK-1. Lane "M" indicates a DNA size marker. Panel B depicts the STM4310 – STM4312 region in strains χ3339 and UK-1. Sequencing of the χ3339 and UK-1 PCR products allowed the target site of the IS200 element before insertion to be identified. The target site was found to be three "T" residues embedded in an A/T rich region. Note that IS200 is a rare example of an insertion sequence that does not have exact repeats at each end. The sequence of the ends shown here in the LT2 sequence are identical to other previously sequence IS200 elements.

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