Figure 4

Selected functional clusters from C. reinhardtii cpDNA. (A) Evidence for co-transcription of the genes rpl 36-rpl 23-rpl 2-rps 19. The gel was loaded with total RNA from wild-type cells, and shows new evidence for co-transcription (see text). The top left lane is an over-exposure of the rpl 36 gel. Transcripts 1 and 2 (3.5 and 3.3 kb) are tricistronic rpl 36-rpl 23-rpl 2, transcript 3A (2.5 kb) is rpl 36-rpl 23, and transcript 3B (2.5 kb) is probably rpl 2-rps 19. Single gene transcripts are labeled "mono". (B) Rearranged functional clusters, which were absent from the inferred common ancestor of C. reinhardtii and C. vulgaris, were identified in C. reinhardtii (genes connected by bold black lines). Cyan lines connect conserved clusters retained from the ancestor cpDNA. The genes are displayed in the coding direction, and from top to bottom relative to their order in the genome. The exception is psb N, which is on the opposite strand relative to other genes shown (psb T-B-N-H). A scale bar of 1 kb is shown below and at the left of each gene cluster.