Table 3 Primer pairs used to amplify by RT-PCR protostome and tunicate family 2 GPCRs

Nematode

CelC13B9.4F1 5' -gatacacgaatttggtgtaatgcc-3'/CelC13B9.4R1 5' -gttcgtgtgaggaccattttcac-3'

C. elegans (Cel)

CelC18B12.2F1 5' -ccattcacattttgcactgcaatt-3'/CelC18B12.2R1 5 -gaaccagagaatagctttgcaaat-3'

Insect

DmeCG13758F 5' -gagattatccgtctcatgca-3'/DmeCG13758R 5' -cgcgttcaacgtggccgtt-3'

D. melanogaste (Dme)

DmeCG8422F 5' -agctgcccaccattatctac-3'/DmeCG8422R 5' -gttctggttaagctgaatggt-3'

A. gambiae (Aga)

DmeCG32843F 5' -gcatcacgctgcacatgaat-3'/DmeCG32843R 5' -cgccgagatgatttcgtatg-3'

AgaP14164F 5' -agcttcgagccggaaattgag-3'/AgaP14164R 5' -ttcgtgatcagcacccacatgat-3'

Tunicate

CinS93F 5'-taccgcttggcgatttctg-3'/CinS93R 5' -aacgacatagagtagcaacga-3'

C. intestinalis (Cin)

CinS372F 5'-aacgacatagagtagcaacga-3'/CinS372R 5'-cggacgaaaatcaaactatg-3'

CinS752F 5'-attgctcacgtgacggtaga-3'/CinS752R 5'-ctccacaaatatttcttgtc-3'

CinS273F 5' -gttttagaaaccggaacatg-3'/CinS273R 5' -agaaaaactgtttcgccggt-3'

Cin50F 5'-gatccataggaagttgaaaag-3'/CinS50R 5' -aatgaaaataatttctccggtt-3'

CinS70F 5'-cagctgtcgactagtaataac-3'/CinS70R 5' -gtatacagagacgatttccttg-3'

CinS5AF 5'-cctggtatttttgatggttat-3'/CinS5AR 5'-attaggtatcatattgtttac-3'

CinS5BF 5' -cctggtttctttgatagacaa-3'/CinS5BR 5' -agttcgtaaagcgttgtagat-3'

Ciona 18S control

Cin18SFwd 5'-cggagaagtttcagcaca-3'/Cin18SRev 5'- agtgtcgcaaacccctgt-3'

18S control

Sb18SFwd 5'-tcaagaacgaaagtcggagg-3'/Sb18SRev 5'-ggacatctaagggcatcaca-3'

β-actin control

SbDebactF3 5'-ggccgcgacctcacagactac-3'/SbDebactR2 5'-accgaggaaggatggctggaa-3'