Figure 3
From: Evolution of secretin family GPCR members in the metazoa
Comparison of the N-terminal end of metazoa family 2 GPCRs. Multiple sequence alignment of the N-terminal domain of the family 2 GPCR receptor genes identified and characterised in protostomes and deuterostomes. Conserved cysteine residues are indicated by ''•'' and the conserved amino acid motifs are boxed. The TM1 domain is annotated. Accession numbers of the human family 2 GPCRs: HsaGLP1R (P43220), HsaCRF1R (P34998) and HsaCALR (P30988). Accession numbers of the protostome family 2 receptor genes: House cricket (Acheta domesticus) Diuretic hormone receptor (AdoDHR, Q16983), Drosophila melanogaster (DmeCG13758, NP_570007; DmeCG8422, NP_610960; DmeCG32843, XP_396046), Anopheles gambiae (AgaP14164, EAA11768), Caenorhabditis. elegans (CelC18B12.2, NP_510496; CelC13B9.4, NP_498465) and Caenorhabditis briggsae (CbrCAE70126, CAE70126; CbrCAE63268, CAE63268). EST data was used to obtain the N-terminal region of the incomplete receptor sequences (Additional file 1). The N-terminal region of Ciona CinS752 was predicted by NIX and that of CinS5A by sequence comparison with CinS5B. Only the clones for which a putative N-terminal domain was identified were included in the analysis. For figure simplicity, an arrow indicates the region of the receptor CbrCAE70126 that was eliminated since it did not align with any other sequences present.