Skip to main content
Figure 3 | BMC Evolutionary Biology

Figure 3

From: Identification of a gonadotropin-releasing hormone receptor orthologue in Caenorhabditis elegans

Figure 3

Expression of Ce-GnRHR. (A). Worm (N2) homogenates were immunoblotted with an affinity purified polyclonal antibody against Ce-GnRHR. The antibody recognized a 46-kDa band, the calculated molecular weight of the GnRHR1 orthologue. The secondary antibody alone did not show immunoreactivity. The molecular weight markers are indicated in kDa on the left. (B). Immunohistochemical localization of Ce-GnRHR was performed on eggs after freeze fracture using a monoclonal antibody against human GnRHR1 (above) or an affinity purified polyclonal antibody against Ce-GnRHR (below). Eggs were similarly stained by both antibodies. No immunoreactivity was detected in eggs probed with the secondary antibody alone. Scale: eggs – 50 μm.

Back to article page