Genetic differentiation in the soil-feeding termite Cubitermes sp. affinis subarquatus: occurrence of cryptic species revealed by nuclear and mitochondrial markers

Table 1 Cubitermes samples and summary of sequence and microsatellite data used to distinguish cryptic species.

Putative species	Colonies	Sites	COII sequences (N = 1)		ITS2 sequences (N = 1)			Microsatellite alleles
			AN	Hap	AN	Hap	N	AR P14 (n)	AR P19 (n)	AR P32 (n)	AR P34 (n)	AR P41 (n)
Cubitermes spA	OKOT7	Okoumé	DQ127300	1	DQ246528	a	26	227–253 (10)	408–412 (2)	160–174 (2)	231–239 (4)	149 (1)
	ROCT16	Rocher	DQ127302	1	DQ246527	a	24
	ROCT42	Rocher	DQ127299	1	DQ246529	a	30
	ROCT14A	Rocher	DQ246540	1	DQ246530	a	6
Cubitermes spB	ROCT14B	Rocher	DQ246542	4	DQ246524	b	12	231–233 (2)	392–408 (4)	160–176 (3)	231–247 (8)	149–155 (3)
	OKOT5	Okoumé	DQ127311	4	DQ246519	b	18
	OKOT26	Okoumé	DQ127312	5	DQ246520	b	24
	CHAT34	Chameau	DQ127309	4	DQ246523	b	26
	CHAT37	Chameau	DQ127310	4	DQ246521	b	48
	CHAT38	Chameau	DQ127308	4	DQ246522	b	33
Cubitermes spC	ROCT17	Rocher	DQ127304	6	DQ246537	c	23	225–245 (8)	404–408 (2)	160–180 (4)	231–245 (5)	149 (1)
	OKOT24	Okoumé	DQ127303	6	DQ246531	c	19
	OKOT31	Okoumé	DQ246543	6	DQ246533	c	24
	CHAT33	Chameau	DQ127307	7	DQ246534	c	19
	ROCT45	Rocher	DQ246544	6	DQ246535	c	20
	ROCT46	Rocher	DQ127306	8	DQ246536	c	34
	CHATX	Chameau	DQ127305	6	DQ246532	c	18
Cubitermes spD	DODTD1	Doda	DQ127301	2	DQ246525	d	24	229–245 (4)	412 (1)	160–178 (2)	233–239 (4)	149 (1)
	DODTD2	Doda	DQ246541	3	DQ246526	d	19
Outgroups	A. longiceps	-	DQ246545	-	DQ246538	-	-	-	-	-	-	-
	C. albotarsalis	-	DQ246546	-	DQ246539	-	-	-	-	-	-	-

GenBank accession numbers (AN) and haplotype names (Hap) for COII and ITS2 sequences and allele ranges (AR) for the five microsatellite loci. N, number of individuals tested for each of the 19 Cubitermes colonies of the four geographical sites and for A. longiceps and C. albotarsalis and n, number of microsatellite alleles found in each locus and putative cryptic species.

ISSN: 2730-7182