A – ML tree of GAPDH protein sequences. ML tree constructed from bacterial GapAB (above dashed line) and eukaryote cytosolic GapC (below dashed line) homologs (271 aligned aa positions). Note the bacterial type GapAB of plant chloroplasts as the result of a transfer from the cyanobacterial ancestor. The dot and the asterisk mark indel events supporting the branching of the parabasalid bacterial type GapAB with Trimastix. Colour coding and labelling as in (1A) plus eukaryotes being labelled in blue. Bootstrap values below 50% are shown where indicative of a potential LGT between bacteria and amitochondriate eukaryotes (Borrelia branching with Trimastix and parabasalids). The tree was rooted with cytosolic GapC homologs for display purposes. B – Indels in the GAPDH protein sequence alignment. Positions 119 to 164 of the GAPDH amino acid alignment are shown here to demonstrate insertions that support the branching of Trimastix and the parabasalids (1 aa – indicated by a dot) and of the spirochaete Borrelia with Trimastix and parabasalids (6–11 aa indicated by an asterisk). The dashed line separates GapAB (top) from GapC (bottom) sequences.