Figure 6

Parsimony tree based on RPB1 sequences showing a clear example of "localized LBA." The green alga Chlamydomonas groups with other green plants and algae in model-based approaches, but is attracted to the diatom Thalassiosira using parsimony. These sequences represent the two "longest branches" within the CTD clade, but do not share any other sub-clade affiliation in the RPB1 tree (Figure 2). This grouping demonstrates that LBA and SBE are not simply global effects, but can cause more localized artifacts as well. Note also that, in parsimony, Glaucocystis groups specifically with Dictyostelium, the shorter branched of the two amoebozoans (Figures 3, 5).