Table 1 Primer sequences used in this study for different mtDNA and nuclear genes; L and H refers to light and heavy strand, respectively, and numbers refers to the 3' position of the Ovis mtDNA sequence.
Primer sequence | Amplicon length |
|---|---|
Cytochrome b gene | |
L14136 5'-GCTTGATATGAAAAACCATCGTTG-3' H14313 5'-TGTGTCGGATGTATAGTGTATTG-3' | 176 bp |
L14310 5'-ATCCTAACAGGCCTATTCCT-3' H14481 5'-CCGATGTTTCATGTTTCTAGGA-3' | 170 bp |
L14475 5'-CGAGGCCTGTACTACGGATC-3' H14650 5'-AACTGAGAATCCGCCTCAG-3' | 174 bp |
L14631 5'-GCTATCCCATACATTGGAAC-3' H14813 5'-GTATARTARGGGTGAAATGG-3' | 181 bp |
L14792 5'-TCCAACAACCCCTCAGGAATTC-3' H14987 5'-TTGATCGTARGATTGCGTATGC-3' | 194 bp |
L14973 5'-CCTCACATCAAACCCGAATG-3' H15159 5'-TCCTCCAATTCATGTGAGTG-3' | 185 bp |
L15152 5'TTCTGAATCCTAGTAGCCGACC-3' H15327 5'-TGCAGTCATCTCCGGTTTACAAGAC-3' | 174 bp |
12 S gene | |
L599 5'-CTCAAAGGACTTGGCGGTGC-3' H673 5'-GAAGATGGCGGTATATAGAC-3' | 73 bp |
L671 5'-TCACCAATCCTTGCTAATAC-3' H805 5'-AATGGCTTTCGTATTAAATT-3' | 133 bp |
L733 5'-AACAAGAGTAAGCTCAATCA-3' H891 5'-CGGTGTGTGCGTGCTTCATG-3' | 157 bp |
28 S gene | |
L28S 5'-GGTCGTCCGACCTGGGTATA-3' H28S 5'-TCTAATCATTCGCTTTACCGGAT-3' | 96 bp |