Late Quaternary loss of genetic diversity in muskox (Ovibos)

Table 2 Primers used in reconstructing cyt b and HV sequences

Target	Primer combination	Primer sequence	Notes
Main cyt b primers	L1 + H1	L1: CCTATACTACGGATCATACA H1: AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA
	L2 + H2	L2: GGGGGAATTCTTCTACTCA H2: GCTGAGAGGAGGTTAGTGA
Additional cyt b primers	L3 + H3	L3: CGAAGCTTGATATGAAAAACCATCGTTG H3: GTAGAATTAGGCAGATACCT	a
	L4 + H4	L4: TCAAACATCTCATCATGATG H4: GTAGAAGAATTACCCCGATG	a
	L5 + H5	L5: CTCCCATTTATCATCGTAGC H5: TTGATCGTAGGATTGCGTAT	a
	L6 + H6	L6: TCACATTAAACCAGAGTGAT H6: GGCATTAGCACCAGGATGAT	a
	L2 + H7	H7: TGGATCCTGTTTCGTGGAGG3	b
	L7 + H1	L7: AAAAAGCTTCCATCCAACATCTCAGCATGATGAAA	c
Main HV region primers	L1 + H1	L1: AAAGGATCCTAAACTACTCCCTGAATT H1: AAAGGATCCATCATGCGTTGTTGCGT
	L2 + H2	L2: AAAGAATTCCCAGTATCAAATCTACC H2: AAAGGATCCGCTTGTGTTACATATGTCT
Additional HV region primers	L3 + H3	L3: CACCCAAAGCTGAAGTTCTA H3: GTTGTTGCGTGTGGAGTAGG	d

Notes. – For certain samples, some primer pairs were more successful than others. Additional primer pairs were applied to a few samples during the course of the study as noted below. The Numt test for sample OMModCl was performed using primer combinations: cyt b L1 + H1, L2 + H2, L7 + H1, HV region L1 + H1, L2 + H2. All primers are shown 5' to 3'.
^aPrimers used to amplify nearly full length cyt b from OMTai39.
^bAdditional primer combination used on OMTai38, 39 and 46.
^cAdditional primer combination used on OMTai14 and 46.
^dAdditional primer combination used on OMTai14, 39, and 46.

ISSN: 2730-7182