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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Diverse spatial, temporal, and sexual expression of recently duplicated androgen-binding protein genes in Mus musculus

Figure 2

Detection of transcripts in the major olfactory epithelium (MOE) of the mouse. RNA purified from the MOE of C3H/HeJ mice was used to make cDNA, which was used as template in PCR reactions that included, separately, the nine Abpa primer sets, the eight Abpbg primer sets and the Gapdh control primer set. The left-hand lane in all gels contained DNA size markers. Panel A: MOE from a male, where the RNA preparation was used to make cDNA without first being treated with RNase-free DNase. The larger band (>1 kb) that appears in most lanes is the amplification product from genomic DNA that contaminated the RNA preparation. Panel B: MOE from a female, where the RNA preparation was treated with RNase-free DNase before it was used to make cDNA. No genomic amplification products appear on this gel.

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