Figure 12
Nonsynonymous versus synonymous substitution rates in the mouse N domain CFG and ABED faces. The nucleotide sequences encoding the CFG and ABED faces of the N1 domain and equivalent regions of the N2 and N3 domains were separated and compared individually. The number of nonsynonymous substitutions per nonsynonymous site (d n ) and the number of synonymous substitutions per synonymous site (d s ) were calculated using the method of Yang and Neilson [46] for pairwise nucleotide comparisons. Plots are shown of d n versus d s for regions comprising (A) the ABED face and, (B) the CFG face, where each data point represents a pairwise comparison of two nucleotide sequences taken from the dataset being examined. The 45° line of equivalence is drawn where d n = d s . (C) Box and whisker plot of d n /d s calculated from pairwise comparisons of all sequences in one dataset against all others in the set. Data derived from sets of rat N1 CFG and ABED faces were also analysed for comparative purposes. Significant differences between CFG and ABED faces for each domain are shown, where '***' is p < 0.0001 (calculated by the Mann-Whitney method).