Table 1 Effect of mutations on kinetic parameters of substrate hydrolysis by single mutations. Data were fitted on equation derived from scheme 1. Only parameters significantly modified in mutated proteins are shown.
Wild type | I161V | G265A | F330Y | G368A | |
|---|---|---|---|---|---|
k2 (s-1) | 19000 | 15000 | 67000 | 30000 | 28000 |
k3 (s-1) | 400 | 154 | 860 | 120 | 180 |
K p (μM) | 180 | - | - | - | 300 |
K L | 1 | - | - | - | 16 |
K LL | 127 | - | 400 | 1150 | - |
a | 4.2 | - | - | - | - |
b | 0.16 | - | - | - | - |
k dec (10-6 s-1) | 108 | 57 | 122 | 61 | 59 |