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Table 1 Substitution patterns of I. perado and I. canariensis ITS sequences from a reconstructed maximum likelihood ancestral ITS sequence of Ilex.

From: Are both sympatric species Ilex perado and Ilex canariensis secretly hybridizing? Indication from nuclear markers collected in Tenerife

  Rate nmC mC Chi2
per 1_0_57 0.045 4/226 (1.77%) 11/174 (6.32%) *
can 28_B_45 0.188 24/226 (10.62%) 37/174 (21.26%) **
can 39_3_53 0.080 11/226 (4.87%) 19/174 (10.92%) *
can 44_4_54 0.061 12/226 (5.31%) 16/174 (9.20%) ns
can 36_1_53 0.090 10/226 (4.42%) 26/174 (14.94% ***
can 90_2_62 0.055 0/226 (0.00%) 2/174 (1.15%) ns
  1. Rate: Kimura 2-parameter distance from the reconstructed ancestral sequence. nmC: Number of C>T substitutions / number of non-methylated cytosines on both DNA strands of the reconstructed ancestral ITS sequence of Ilex. mC: Number of C>T substitutions / number of methylated cytosines on both DNA strands of the reconstructed ancestral ITS sequence of Ilex. The corresponding ratios of C>T substitutions are indicated between brackets. Chi2: Chi-square homogeneity test between expected and observed C>T substitutions at methylated cytosines (ns: non significant; *, ** and ***: significant at 0.05, 0.01 and 0.001 levels, respectively).