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Table 1 Substitution patterns of I. perado and I. canariensis ITS sequences from a reconstructed maximum likelihood ancestral ITS sequence of Ilex.

From: Are both sympatric species Ilex perado and Ilex canariensis secretly hybridizing? Indication from nuclear markers collected in Tenerife

 

Rate

nmC

mC

Chi2

per 1_0_57

0.045

4/226 (1.77%)

11/174 (6.32%)

*

can 28_B_45

0.188

24/226 (10.62%)

37/174 (21.26%)

**

can 39_3_53

0.080

11/226 (4.87%)

19/174 (10.92%)

*

can 44_4_54

0.061

12/226 (5.31%)

16/174 (9.20%)

ns

can 36_1_53

0.090

10/226 (4.42%)

26/174 (14.94%

***

can 90_2_62

0.055

0/226 (0.00%)

2/174 (1.15%)

ns

  1. Rate: Kimura 2-parameter distance from the reconstructed ancestral sequence. nmC: Number of C>T substitutions / number of non-methylated cytosines on both DNA strands of the reconstructed ancestral ITS sequence of Ilex. mC: Number of C>T substitutions / number of methylated cytosines on both DNA strands of the reconstructed ancestral ITS sequence of Ilex. The corresponding ratios of C>T substitutions are indicated between brackets. Chi2: Chi-square homogeneity test between expected and observed C>T substitutions at methylated cytosines (ns: non significant; *, ** and ***: significant at 0.05, 0.01 and 0.001 levels, respectively).