The four fugu scaffolds analyzed and visualized using the GESTALT Workbench. The RD genes are aligned by the position of the proximal promoter. For each scaffold, graphs are shown of the CpG contrast values (observed/expected, typically ~0.5 for the fugu genome), G+C percentage (green – below 43%, blue – between 43% and 50%, red – above 50%), interspersed repeats (SINEs in red, LINEs in green, DNA and LTR elements in brown, other repeats in purple), and gene annotations. Genes and repeats displayed above each black midline are in the forward strand, while those displayed under the midline are in the reverse strand of the sequence. All scaffolds are shown at a resolution of 200 bp/pixel, except for scaffold 835, which includes the tiny FrRUNT gene. For clarity, only the last 17 kb of this scaffold are shown at 20 bp/pixel. The much longer scaffold scaffold 183 is also shown truncated at 182 kb; the LCK locus has been studied in detail elsewhere .