Function and evolution of the serotonin-synthetic bas-1 gene and other aromatic amino acid decarboxylase genes in Caenorhabditis

Table 3 Synonymous vs. non-synonymous codon substitution between C. elegans and C. briggsae AADC orthologs and bas-1-like paralogs.

Ce, Cb AADCs compared	Codons	pS	pN	K_A/K_S
bas-1	521	0.68	0.07	0.039
C05D2.3, F12A10.3, bas-1*	521	0.71 ± 0.03	0.26 ± 0.02	0.148 ± 0.044
ZK289.2	833	0.71	0.09	0.043
C09G9.4	507	0.74	0.12	0.037
tdc-1 full length	626	0.79	0.03	NA
tdc-1 core	474	0.82	0.02	NA
tdc-1 N, C terminals	152	0.71	0.07	0.035

Nucleotide alignments of C. elegans and C. briggsae genes were analyzed by SNAP software (see Methods). Only the C. elegans member of the ortholog pair is named. pS = proportion of observed/potential synonymous substitutions; pN = proportion of observed/potential nonsynonymous substitutions. NA – not applicable (cannot be calculated when pS > 0.75). *Includes all pairwise comparisons (n = 5) except C.e. vs. C.b. bas-1. Values are mean ± SD (strict statistical comparison with other values is not intended, as K_A/K_S values are not distributed normally).

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