Function and evolution of the serotonin-synthetic bas-1 gene and other aromatic amino acid decarboxylase genes in Caenorhabditis

Table 1 Pairwise BLAST comparisons with C. elegans AADCs.

	C05D2.4	C05D2.3	F12A10.3*	K01C8.3	ZK829.2	C09G9.4	Ce GAD
C05D2.4 (bas-1)	Score %Id / %Sim	-	-	-	-	-	-
C05D2.3	1674 59 / 75	-	-	-	-	-	-
F12A10.3*	1756 60 / 77	1793 63 / 78	-	-	-	-	-
K01C8.3 (tdc-1)	970 37 / 57	844 34 / 54	733 33 / 51	-	-	-	-
ZK829.2	583 29 / 47	541 27 / 46	559 27 / 49	1147 44 / 66	-	-	-
C09G9.4	224 22 / 40	150 18 / 38	190 19 / 40	272 22 / 42	275 23 / 44	-	-
Ce GAD (unc-25)	210 22 / 38	164 20 / 36	216 23 / 37	299 25 / 43	250 24 / 41	129 20 / 40	-
Dm DDC	1095 41 / 60	909 36 / 56	984 38 / 58	1390 50 / 69	883 37 / 57	256 22 / 42	328 24 / 40
Hs DDC	1067 41 / 60	912 36 / 55	949 38 / 58	1458 55 / 73	935 39 / 59	233 20 / 42	322 26 / 44
Dm HisDC	996 39 / 57	816 33 / 53	807 32 / 54	1348 52 / 70	910 40 / 59	278 23 / 42	339 26 / 44
Hs HisDC	988 38 / 56	802 33 / 53	876 34 / 56	1290 48 / 69	920 39 / 59	231 21 / 42	306 26 / 42
Dm G30446	971 38 / 57	845 34 / 53	686 30 / 50	1671 64 / 77	1008 40 / 62	254 20 / 44	n.s.
Dm AMD	961 38 / 57	799 35 / 53	668 36 / 53	1124 44 / 63	741 33 / 51	201 20 / 40	278 25 / 41
Dm G30445	837 34 / 52	797 35 / 53	814 33 / 53	1407 55 / 71	905 39 / 57	259 22 / 42	n.s.
Cr TrpDC	743 30 / 51	674 28 / 50	735 30 / 52	916 37 / 59	269 31 / 51	272 24 / 43	333 25 / 42
Dm GAD	226 22 / 39	207 21 / 38	232 22 / 41	374 27 / 43	226 22 / 41	99 25 / 55	1146 44 / 64
Hs GAD67	215 22 / 36	173 19 / 36	192 21 / 36	324 24 / 44	228 24 / 41	86 16 / 41	1535 56 / 73

Comparisons of bas-1/C05D2.4 and other pyridoxal-phosphate dependent decarboxylase amino acid sequences were made using "BLAST 2 Sequences" [version 2.2.6, http://www.ncbi.nlm.nih.gov/blast/bl2seq/bl2.html [73]; Settings (largely default): Matrix – BLOSUM62, Open gap penalty – 11, extension gap penalty – 1, low complexity filtering – OFF). As shown in the table above, on the top line, each comparison shows the blast score; below is the percent identity and percent similarity for the 'alignable' sequence. The highest scoring match (excluding among C. elegans AADCs) is indicated in bold. Sequences in the left column are arranged in order of blast score in comparison to C05D2.4 C. elegans AADCs are indicated by their predicted gene designation: C05D2.4, C05D2.3, F12A10.3, K01C8.3, ZK829.2 and C09G9.4. Abbreviations: Ce – C. elegans, Cr – Caranthus roseus (periwinkle plant), Dm – Drosophila melanogaster, Hs – Homo sapiens, DDC – dopa decarboxylase, HisDC – histidine decarboxylase, AMD – Alpha-methyl dopa hypersensitive protein, TrpDC – tryptophan decarboxylase, GAD – glutamate decarboxylase. *An amino acid sequence for F12A10.3 was generated from cDNA sequence by introducing 2 frameshifts to preserve AADC homology in the predicted aa sequence merely for sake of comparison to other AADC's (see text); this sequence is different from predicted sequences in found in Genbank and Wormbase which are based on incorrect cDNA predictions.

ISSN: 2730-7182