Figure 6

Phylogenetic trees of AADC protein and nucleotide sequences. Trees were made from sequences aligned with CLUSTALW. Species and gene names are abbreviated as listed in the legend for Table 1. (A) The single minimum-length tree resulting from a heuristic search using parsimony from alignments of core protein sequences (531 characters) of selected C. elegans, C. briggsae, Human and Drosophila AADCs. C. roseus (periwinkle plant) TrpDC was used as an outgroup. Branch lengths are indicated, with bootstrap values using the same search conditions (1000 replicates) in parentheses. The search used the tree-bisection-reconnection (TBR) branch-swapping algorithm; characters were equally weighted. An identical tree topologically was obtained by a branch-and-bound search. C. elegans F12A10.3 was excluded from this analysis since it lacks a functional protein sequence (see Fig. 7 and text). Trees determined by distance methods were similar, but rearranged some of branches with low bootstrap values in the tree shown. (B) The single minimum-length tree resulting from a heuristic search using parsimony (same settings as above) of nucleotide sequence alignments (1608 characters) from a subset of AADCs above, with the addition of C. elegans F12A10.3. Dm DDC was used as an outgroup. Branch lengths and bootstrap values using the same search conditions (1000 replicates) are shown as in A. An identical tree topologically was obtained by a branch-and-bound search.