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Table 1 Effect of CoA linkage on fitness for selection for disulfide-linked versus amide-linked CoA resins (compare structures in figure 1).

From: The tyranny of adenosine recognition among RNA aptamers to coenzyme A

RNA

Percent of input RNA eluted from...

 

Amide linked CoA resin

Disulfide linked CoA resin

70CoA-min52

34%

28%

70SA selected pool

20%

70%

  1. Transcripts of aptamer 70CoAmin-52 and of the 70SA pool (500 pmole) were loaded onto each of the indicated CoA resins, washed with 10 column volumes of binding buffer and eluted with 5 mM CoA.
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BMC Ecology and Evolution

ISSN: 2730-7182

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