Figure 3

Elution specificities of selected aptamers. A) The indicated aptamers were loaded onto the disulfide-linked CoA affinity resin. The percentage of input RNA eluted with 5 mM CoA are plotted on the graph. Prior to the elution, non-specifically-bound RNAs were removed with 10 column volumes of binding buffer (gray bars) or with 10 volumes of 1 mM adenosine (black bars). B) The aptamers indicated below each set of columns were bound to the disulfide-linked CoA resin, then washed with five volumes each of binding buffer, then 1 mM analog (gray bars), and finally of 5 mM CoA (black bars). Analogs were either pantotheine alone (P), AMP alone (A), AMP and pantotheine together (AP), or CoA (C). Percent elution is normalized to total RNA eluted in five "analog" fractions plus five "CoA" washes for ease of comparison. Under the conditions used in this assay, all of the aptamers gave approximately 50% total elution.